| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bordetella avium | TTTCTCGCCAGCCAGCAT | ACCCAGTTGGCTGATGGTG | 59.65 | 59.92 | 276 | 149 |
100.00%
|
100.00%
|
| Primer 2 | Bordetella avium | ACTTTCTGGCTGCGCTGT | TTCGCCTATCACGAGCACC | 59.89 | 59.86 | 224 | 148 |
100.00%
|
100.00%
|
| Primer 3 | Bordetella avium | ACTTTCTGGCTGCGCTGT | ACACCGGTTCGCCTATCAC | 59.89 | 59.78 | 231 | 148 |
100.00%
|
100.00%
|
| Primer 4 | Bordetella avium | ACTTTCTGGCTGCGCTGT | TGCCCTGGAGCGTACTTTC | 59.89 | 59.70 | 282 | 148 |
100.00%
|
100.00%
|
| Primer 5 | Bordetella avium | GTACGCTCAGCCGTTTTGG | TGCGAGACCAGGACAAAGG | 59.50 | 59.63 | 178 | 145 |
100.00%
|
100.00%
|
| Primer 6 | Bordetella avium | ACTTTCTGGCTGCGCTGT | TCGCAGACCTCGTATTGCC | 59.89 | 59.86 | 190 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Bordetella avium | ACTTTCTGGCTGCGCTGT | GGTTCGCCTATCACGAGCA | 59.89 | 59.86 | 226 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Bordetella avium | ACCGAAGTGATGAGCGCTG | TCGAGCAGTTTGTCGGCA | 60.45 | 59.58 | 299 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Bordetella avium | TCCCGAACGCTTGCAGAT | TGCCGTCGATGTTCAGCA | 59.34 | 59.66 | 188 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Bordetella avium | TTTCGCGTTCGTTGCTCC | GGACGCATGTGCAAACTCC | 59.07 | 59.79 | 219 | 138 |
100.00%
|
100.00%
|